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Substrates

Substrates

Substrates are laboratory reagents, chemicals, and consumables, and kits used to support or enhance biomedical, pharmaceutical, clinical, food science, environmental, and other research and testing laboratory processes. Products include enzymes, inhibitors, acids, bases, buffers, antibodies, and chromatography supplies.
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1 – 15 of 249 results
1

Thermo Scientific™ LanthaScreen™ Tb-DUB Substrate

Deubiquitinating enzymes (DUBs) play housekeeping functions by maintaining pools of active ubiquitin, and regulatory function by rescuing specific target proteins from degradation by the proteasome.

2

Thermo Scientific™ beta-Galactosidase Assay Reagent

Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.

Substrate Beta-Gal Substrate
Assay Reporter Enzyme
Substrate Properties Chemical Substrate
Content And Storage Store at -20°C.
Format 96-well Plate
Product Type Pierce beta-Galactosidase Assay Reagent
For Use With (Equipment) Microplate Reader
Form Liquid
Label or Dye Enzyme Labeled
Target Enzyme Beta-Galactosidase
For Use With (Application) Absorbance
Product Line Pierce™
Detection Method Colorimetric
3

Thermo Scientific Chemicals Cathepsin K Substrate (fluorogenic)

For cellular and molecular biology applications

Form Solid
Molecular Weight (g/mol) 578.65
Color White
Substrate Type Cathepsin Substrate
Storage Requirements -30° to -10°C
For Use With (Application) A sensitive fluorogenic substrate for cathepsin K
4

Thermo Scientific™ PI:PS Lipid Kinase Substrate

Specifically optimized for use with the Class III PI3 kinase (PIK3C3 (hVPS34)) and Type III PI4 lipid kinases, this substrate can also be used as a general substrate for the Class I lipid kinases.

7

Thermo Scientific™ GFP-STAT3

STAT3 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for the Janus family of tyrosine kinases.

9

Invitrogen™ Alexa Fluor™ 647-Poly-GT Peptide Substrate

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.

Shipping Condition Dry Ice
Excitation/Emission 340/665 nm
Substrate Random (Glu:Tyr) polymer in a ratio of 4:1
Substrate Properties Peptide-Based Substrate
Form Liquid
Label or Dye Alexa Fluor™ 647
Color Far-Red
Target Enzyme Tyrosine Kinases
Substrate Type Kinase Substrate
Validated Kinases EGFR
Technique TR-FRET
Detection Method Fluorescence
12

Thermo Scientific™ Vivid™ OOMR Substrate

Vivid™ Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs.

Shipping Condition Approved for shipment on Wet or Dry Ice
Substrate OOMR
Substrate Properties Chemical Substrate
Content And Storage Vivid™ CYP450 Screening kits include:
• Vivid™ CYP450 Fluorogenic substrates (also available separately)
• BACULOSOMES™ CYP450 reagents
• Fluorescent standards
• NADPH-regeneration system

Store reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)
Substrate Type Cytochrome P450 Substrate
Product Line VIVID™
Detection Method Fluorescence
13

Thermo Scientific™ ZIPtide Substrate

A peptide substrate for the Ca/Calmodulin-dependent protein kinase (CaM) family including CAMK1 and DAPK1.

15

Invitrogen™ PI(5)P Lipid Kinase Substrate

The PI(5)P Lipid Kinase Substrate has been specifically optimized for use with Type II PIP kinases (e.g., PIP4K2A)

Shipping Condition Dry Ice
Substrate Phosphatidylinositol 5-phosphate (PI(5)P)
Content And Storage Phosphatidylinositol 5-phosphate (PI(5)P) in 30 mM HEPES pH 7.5 buffer.
Form Powder
Substrate Type Kinase Substrate
Product Line Invitrogen™

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