Restriction Enzymes
Restriction Enzymes
- (1)
- (62)
- (616)
- (579)
- (80)
- (2)
- (1)
- (2)
- (1)
- (1)
- (4)
- (2)
- (2)
- (2)
- (1)
- (1)
- (2)
- (1)
- (4)
- (2)
- (1)
- (3)
- (3)
- (3)
- (7)
- (1)
- (5)
- (2)
- (1)
- (4)
- (2)
- (2)
- (2)
- (1)
- (1)
- (4)
- (5)
- (1)
- (1)
- (2)
- (2)
- (1)
- (2)
- (1)
- (2)
- (3)
- (2)
- (2)
- (2)
- (2)
- (1)
- (2)
- (2)
- (1)
- (1)
- (1)
- (1)
- (1)
- (2)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (2)
- (2)
- (1)
- (1)
- (2)
- (1)
- (3)
- (2)
- (2)
- (1)
- (2)
- (1)
- (3)
- (1)
- (2)
- (2)
- (1)
- (2)
- (1)
- (1)
- (1)
- (1)
- (2)
- (1)
- (1)
- (1)
- (4)
- (3)
- (2)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (2)
- (2)
- (2)
- (2)
- (1)
- (1)
- (2)
- (2)
- (1)
- (6)
- (4)
- (2)
- (2)
- (1)
- (1)
- (1)
- (1)
- (2)
- (2)
- (1)
- (1)
- (2)
- (2)
- (1)
- (3)
- (2)
- (4)
- (3)
- (2)
- (1)
- (2)
- (2)
- (2)
- (2)
- (1)
- (2)
- (2)
- (1)
- (2)
- (2)
- (2)
- (1)
- (2)
- (2)
- (2)
- (1)
- (7)
- (1)
- (1)
- (3)
- (1)
- (2)
- (2)
- (1)
- (1)
- (1)
- (4)
- (2)
- (2)
- (1)
- (2)
- (3)
- (2)
- (1)
- (2)
- (1)
- (2)
- (1)
- (4)
- (7)
- (1)
- (1)
- (5)
- (3)
- (1)
- (2)
- (2)
- (2)
- (2)
- (2)
- (2)
- (2)
- (1)
- (2)
- (6)
- (2)
- (2)
- (2)
- (2)
- (2)
- (2)
- (1)
- (1)
- (2)
- (3)
- (2)
- (1)
- (4)
- (4)
- (2)
- (2)
- (4)
- (4)
- (2)
- (2)
- (2)
- (1)
- (2)
- (2)
- (3)
- (2)
- (3)
- (2)
- (2)
- (2)
- (1)
- (1)
- (1)
- (1)
- (7)
- (6)
- (5)
- (2)
- (1)
- (9)
- (2)
- (1)
- (1)
- (1)
- (2)
- (2)
- (3)
- (3)
- (2)
- (2)
- (2)
- (1)
- (2)
- (2)
- (2)
- (2)
- (2)
- (2)
- (1)
- (2)
- (1)
- (3)
- (1)
- (1)
- (2)
- (2)
- (2)
- (1)
- (1)
- (2)
- (6)
- (1)
- (1)
- (2)
- (2)
- (4)
- (4)
- (2)
- (4)
- (2)
- (2)
- (2)
- (4)
- (5)
- (2)
- (2)
- (2)
- (4)
- (1)
- (1)
- (3)
- (1)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (3)
- (2)
- (7)
- (2)
- (1)
- (1)
- (1)
- (1)
- (3)
- (1)
- (1)
- (2)
- (1)
- (2)
- (2)
- (1)
- (2)
- (2)
- (3)
- (1)
- (3)
- (1)
- (1)
- (2)
- (2)
- (2)
- (2)
- (2)
- (1)
- (2)
- (1)
- (7)
- (2)
- (1)
- (7)
- (3)
- (2)
- (2)
- (2)
- (9)
- (598)
- (2)
- (21)
- (25)
- (92)
- (562)
Filtered Search Results
Thermo Scientific™ FastDigest EcoRI
The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | EcoR I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest HindIII
The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Hind III |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ EcoRI (10 U/μL)
The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.
Concentration | 10 U/μL |
---|
Thermo Scientific™ FastDigest SacI
The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | SacI |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 3' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ HindIII (10 U/μL)
The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.
Concentration | 10 U/μL |
---|
Thermo Scientific™ FastDigest AluI
Cut at AG^CT sites with Thermo Scientific™ FastDigest™ AluI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AluBI).
Invitrogen™ Anza™ 32 ApaI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ ATP Solution (100 mM)
Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Thermo Scientific™ Bovine Serum Albumin (BSA)
Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.
Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)
Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).
Thermo Scientific™ TBE Buffer (Tris-borate-EDTA) (10X)
Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA).
Thermo Scientific™ FastDigest NotI
The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Not I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest BpiI (IIs class)
Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I).
Thermo Scientific™ FastDigest DpnI
Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Dpn I |
Methylation Sensitivity | Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | Blunt |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest NheI
Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Nhe I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |