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Thermo Scientific™ TBE Buffer (Tris-borate-EDTA) (10X)

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA).

Brand:  Thermo Scientific™ B52

33.26 EUR valid until 2025-03-29
Use promo code "24111" to get your promotional price.


Product Code. 10130740

  • € 49.89 / 1 L

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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price

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Description

Description

TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X powder, sc-296647) is recommended when looking at enzymatic applications for the DNA sample.

Usage Recommendations

  • Use fresh 1X TBE both for the gel and for the electrophoresis run
  • To prepare 1X TBE buffer, add 100mL of 10X TBE buffer to 900mL of deionized water and mix well

Quality Control

  • The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

1X Composition

  • 89mM Tris, 89mM boric acid, 2mM EDTA, pH of 10X TBE: 8.3

Recommended for:

Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for electrophoresis of RNA and DNA fragme.

Note:

Double-stranded linear nucleic acid molecules migrate about 10% slower in TBE buffer than in TAE buffer.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

10X
1 L
TBE Buffer
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Lot Number Certificate Type Date Catalog Number
Lot Number2841093 Certificate TypeCertificate of Analysis Date31/01/2025 Catalog NumberB52
Lot Number2800533 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberB52
Lot Number2819508 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberB52
Lot Number2803927 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberB52
Lot Number2856479 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberB52
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Thermo Scientific™ TBE Buffer (Tris-borate-EDTA) (10X) > 1L

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