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IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.

Brand: IBA Lifesciences 5-4705-001

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Product Code. 15643818

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Beschreibung

pYSG-IBA yeast expression vectors

The pYSG-IBA vector allows the high-level expression of proteins in yeast.

The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
The expressed recombinant protein will be localized in the cytoplasm.

High-level expression in yeast with high copy number
Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
Tightly regulated expression due to Cu⁺⁺-inducible CUP1 promoter
Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
URA3 auxotrophy marker for selection after transformation

Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Spezifikation

Format	Liquid
Inducing Agent	Copper sulfate
pH	8
Product Type	Acceptor Vector for yeast expression
Content And Storage	2°C to 8°C for frequent usage, -20°C for long-term storage
Concentration	250 ng/μL
Vector	pYSG-IBA 105
For Use With (Application)	Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins

For Use With (Equipment)	StarGate™ System, Yeast Host Cells
Marker Gene	leu2-d and URA3
Promoter	CUP1
Storage Buffer	20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Protein Tag	Twin-Strep-tag for N-terminal
Quantity	5 μg
Color	Clear

Produktvorschläge

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Dokumentation

Product Information

Manuals

Manual StarGate 5-4705-001 File size: 2.3 MB

Manual StarGate-Direct-Transfer-Cloning 5-4705-001 File size: 1.7 MB

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IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression > StarGate pYSG Acceptor Vector: pYSG-IBA105; Twin-Strep-tag™ for N-terminal

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