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IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.

€ 220.00 - € 266.00

Specifications

Format	Liquid
For Use With (Equipment)	StarGate™ System, Yeast Host Cells
Inducing Agent	Copper sulfate
Marker Gene	leu2-d and URA3
pH	8

View More Specs

Products 21

Product Code	Brand	Vector	Price	Quantity & Availability
Product Code	Brand	Vector	Price	Quantity & Availability
15613808 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4600-001	pYSG-IBA wt1	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15623808 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4603-001	pYSG-IBA 3	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15633808 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4605-001	pYSG-IBA 5	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15643808 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4623-001	pYSG-IBA 23	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15653808 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4625-001	pYSG-IBA 25	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15663808 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4633-001	pYSG-IBA 33	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15673808 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4643-001	pYSG-IBA 43	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15683808 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4645-001	pYSG-IBA 45	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15693808 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4662-001	pYSG-IBA 62	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15603818 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4663-001	pYSG-IBA 63	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15613818 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4664-001	pYSG-IBA 64	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15623818 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4665-001	pYSG-IBA 65	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15633818 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4703-001	pYSG-IBA 103	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15643818 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4705-001	pYSG-IBA 105	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15653818 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4723-001	pYSG-IBA 123	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15663818 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4743-001	pYSG-IBA 143	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15673818 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4745-001	pYSG-IBA 145	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15683818 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4762-001	pYSG-IBA 162	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15693818 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4764-001	pYSG-IBA 164	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15603828 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4767-001	pYSG-IBA 167	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15613828 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4768-001	pYSG-IBA 168	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

Description

pYSG-IBA yeast expression vectors

The pYSG-IBA vector allows the high-level expression of proteins in yeast.

The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
The expressed recombinant protein will be localized in the cytoplasm.

High-level expression in yeast with high copy number
Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
Tightly regulated expression due to Cu⁺⁺-inducible CUP1 promoter
Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
URA3 auxotrophy marker for selection after transformation

Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

Format	Liquid
Inducing Agent	Copper sulfate
pH	8
Product Type	Acceptor Vector for yeast expression
Content And Storage	2°C to 8°C for frequent usage, -20°C for long-term storage
Quantity	5 μg
For Use With (Application)	Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins

For Use With (Equipment)	StarGate™ System, Yeast Host Cells
Marker Gene	leu2-d and URA3
Promoter	CUP1
Storage Buffer	20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Concentration	250 ng/μL
Color	Clear

Safety and Handling

missing translation for 'shelfLife' : 12 months

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IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression

Specifications

Description

Specifications

Safety and Handling

Documents