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Thermo Scientific™ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
Brand: Thermo Scientific™ F160L
358.60 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols, and others.
Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria, and yeast. The kit includes a complete set of optimized reagents and detailed protocols, making it an ideal choice for amplification of plant DNA.
Features
- Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
- Available Dilution & Storage protocol allows several PCR reactions from one sample
- Specially engineered Phire Hot Start II DNA Polymerase for extremely short PCR protocol times
- Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel
Applications
- Genotyping
- Transgene detection
- Knockout analysis
- Sequencing
Notes
- Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
Specifications
| • 2X Phire Plant Direct PCR Master Mix, 10 x 1.25 mL • Dilution Buffer, 2 x 5 mL • 25 μM Control Primer Mix, 40 μL • Nuclease-free water, 10 x 1.25 mL • O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications) Store at -20°C. |
|
| Direct PCR | |
| Fast | |
| Wet or Dry Ice | |
| 2X | |
| 1250 Reactions | |
| SuperMix or Master Mix | |
| DNA |
| Medium | |
| Phire Hot Start II DNA Polymerase | |
| Direct PCR Master Mix | |
| Genotyping, Hot-start PCR | |
| Built-In Hot Start | |
| Blunt | |
| Plant |
For Research Use Only. Not for use in diagnostic procedures.