Direct PCR Reagents and Kits
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Direct PCR Reagents and Kits
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Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
Shipping Condition | Wet or Dry Ice |
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GC-Rich PCR Performance | Medium |
Includes Label or Dye | Yes |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Genotyping,Hot-start PCR |
PCR Method | Direct PCR |
Polymerase | Phire Hot Start II DNA Polymerase |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Tissue |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
First Strand cDNA Synthesis Kit
Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates.
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Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
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Format | Spin Column |
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Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
Shipping Condition | Room Temperature |
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Format | Kit |
Sample Type | PCR Products |
Isolation Technology | Silica Spin Column |
Run Time | 10 min. |
Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit Reaction Mix (5X)
Thermo Scientific™ Maxima™ 5X Reaction Mix is a component of the Maxima First Strand cDNA Synthesis Kits for RT-qPCR (K1671/K1672/K1641/K1642) and may be purchased separately. The 5X Reaction Mix is comprised of reaction buffer, dNTPs, oligo (dT)18 and random hexamer primers.
Thermo Scientific™ Phusion Human Specimen Direct PCR Kit
Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.
Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit
ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.
Shipping Condition | Room Temperature |
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Target | DNA from Enzymatic Reactions (e.g. PCR) |
Content And Storage | The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. |
Format | Kit |
Product Type | PCR Clean-Up Kit |
Sample Type | PCR Products |
Isolation Technology | ChargeSwitch™ Chemistry |
Run Time | 5 min |
For Use With (Application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
High-throughput Compatibility | High-throughput Compatible |
Product Line | ChargeSwitch™ |
Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents
Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Frozen, Dry Ice |
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GC-Rich PCR Performance | High |
Content And Storage | Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C) |
Form | Liquid |
Isolation Technology | Direct cell lysis |
Kit Type | Direct Cell Lysis RT-qPCR |
Green Features | Less hazardous, less waste |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | Yes |
PCR Method | 2-step RT-qPCR |
Detection Method | TaqMan |
Target | cDNA |
Format | Kit |
Sample Type | Mammalian Cells |
Reaction Speed | 5 min. (Fast) |
Label or Dye | ROX |
Purification Target | RNA |
Shelf Life | 1 Year |
Starting Material | 10-100,000 Cells |
Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2X |
Green Features | Fewer resources used and less waste, Less hazardous |
Concentration | 2X |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | High-throughput Compatible |
PCR Method | 2-step RT-qPCR |
Detection Method | SYBR |
Format | Tube |
Product Type | Fast Advanced Cells-to-Ct Kit |
Sample Type | Mammalian Cells |
Reaction Speed | 7 min. |
Reagent Type | Cell Lysis |
Purification Target | Lysate |
Starting Material | 10-100,000 Cells |
Product Line | Cells-to-CT™ |
Thermo Scientific™ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
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Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Plant |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Genotyping,Hot-start PCR |
PCR Method | Direct PCR |
Applied Biosystems™ SNaPshot™ Multiplex Kit
The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).
Product Type | Multiplex Kit |
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For Use With (Equipment) | 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers |
Sample Type | DNA |
Technique | SNP Genotyping (Fragment Analysis-Based), Primer Extension |
Product Line | SNaPshot™ |
Detection Method | Primer-probe |
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
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This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More About the Greener Choice Program
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
GC-Rich PCR Performance | High |
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Format | Tube |
Product Type | 1-Step qPCR kit |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Mammalian Cells |
Reaction Speed | 7 min. |
Reagent Type | Cell Lysis |
Green Features | Fewer resources used and less waste, Less hazardous |
Starting Material | 10-100,000 Cells |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | Cells-to-CT™, TaqMan™ |
Detection Method | TaqMan |
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2X |
Green Features | Fewer resources used, less waste, less hazardous |
Concentration | 2X |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | High-throughput Compatible |
PCR Method | 2-step RT-qPCR |
Detection Method | TaqMan |
Format | Tube |
Product Type | Fast Advanced Cells-to-Ct Kit |
Sample Type | Mammalian cells |
Reaction Speed | 7 min. |
Reagent Type | Cell Lysis |
Purification Target | Lysate |
Starting Material | 10-100,000 Cells |
Product Line | Cells-to-CT™ |