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Life Science Buffers

Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

  • Temperature
  • Desired or target pH
  • Buffer toxicity (to the system)
  • Buffer interactions with other system components

 

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1 – 15 of 36 results
1

TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™

Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT

Promotions are available
EDGE
Name Note 50X Solution
Concentration or Composition (by Analyte or Components) Tris (24%), Acetic Acid (5.0%), and EDTA (<2%).
CAS 6850-28-8
Protease Protease free
Physical Form Liquid
Grade Electrophoresis
Synonym TAE
Purity Grade Notes DNase-, RNase- and Protease-Free
DNase DNase free
Recommended Storage RT
Filtered Through Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol Gray
3

TAE Buffer, Tris-Acetate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents

Promotions are available
Name Note 10X Solution
Concentration or Composition (by Analyte or Components) Tris (<5.0%), Acetic Acid (1.0%), and EDTA (<1%).
CAS 77-86-1
Protease Protease free
Physical Form Liquid
Chemical Name or Material Tris-Acetate-EDTA
Grade Electrophoresis
Synonym TAE
Purity Grade Notes DNase-, RNase- and Protease-Free
DNase DNase free
Recommended Storage RT
Filtered Through Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol Gray
4

TBE Buffer, Tris-Borate-EDTA, 1X Solution, Electrophoresis, Fisher BioReagents

Promotions are available
Name Note 1X Solution
CAS 7732-18-5
Protease Protease free
Color Colorless
Physical Form Liquid
pH 8.2 to 8.4
Chemical Name or Material Tris-Borate-EDTA
Grade Electrophoresis
Synonym TBE
DNase DNase free
Recommended Storage RT
Filtered Through Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol Gray
5

Tris-Borate-Edta Buffer, for Electrophoresis, (10X Concentration), Fisher Chemical™

CAS: 610769-35-2 Molecular Formula: C14H30BN3O14 Molecular Weight (g/mol): 475.211 MDL Number: 4679 InChI Key: OSBLTNPMIGYQGY-UHFFFAOYSA-N PubChem CID: 21257725 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid SMILES: B(O)(O)O.C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O

PubChem CID 21257725
CAS 610769-35-2
Molecular Weight (g/mol) 475.211
MDL Number 4679
SMILES B(O)(O)O.C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid
InChI Key OSBLTNPMIGYQGY-UHFFFAOYSA-N
Molecular Formula C14H30BN3O14
6

Tris-Glycine-SDS, 10X Solution (Electrophoresis), Fisher BioReagents™

Promotions are available
EDGE
Color Undesignated
Physical Form Liquid
Chemical Name or Material Tris-Glycine-SDS
Grade Electrophoresis
Identification Pass Test
DNase DNase free
Filtered Through Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol Gray
Name Note 10X Solution
Concentration or Composition (by Analyte or Components) 0.25M Tris Base, 1.92M Glycine, and 1.0% (w/v) SDS.
CAS 151-21-3
Protease Protease free
Synonym TG-SDS
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
7

Thermo Scientific Chemicals BICINE, Electrophoresis Grade, 99+%

CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: N,N-Bis(2-hydroxyethyl)glycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: [Na+].OCCN(CCO)CC([O-])=O

PubChem CID 8761
CAS 150-25-4
Molecular Weight (g/mol) 185.16
ChEBI CHEBI:40957
MDL Number MFCD00004295
SMILES [Na+].OCCN(CCO)CC([O-])=O
Synonym N,N-Bis(2-hydroxyethyl)glycine
IUPAC Name 2-[bis(2-hydroxyethyl)amino]acetic acid
InChI Key MFBDBXAVPLFMNJ-UHFFFAOYSA-M
Molecular Formula C6H12NNaO4
8

Tris-Glycine, 10X Solution (Electrophoresis), Fisher BioReagents™

Color Undesignated
Physical Form Liquid
Chemical Name or Material Tris-Glycine
Grade Electrophoresis
DNase DNase free
Filtered Through Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol Gray
Name Note 10X Solution
Concentration or Composition (by Analyte or Components) Components: Tris (2-3%) and Glycine (13-14%).
0.25M Tris Base and 1.92M Glycine.
CAS 56-40-6
Protease Protease free
Synonym TG
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
9

Tricine, Electrophoresis Grade, 99%, Thermo Scientific Chemicals

CAS: 5704-04-1 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N Synonym: N-(Trishydroxymethylmethyl)glycine PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO

PubChem CID 79784
CAS 5704-04-1
Molecular Weight (g/mol) 179.17
ChEBI CHEBI:39063
MDL Number MFCD00004277
SMILES C(C(=O)O)NC(CO)(CO)CO
Synonym N-(Trishydroxymethylmethyl)glycine
IUPAC Name 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
InChI Key SEQKRHFRPICQDD-UHFFFAOYSA-N
Molecular Formula C6H13NO5
10

Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane, Electrophoresis Grade, 99.5%

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
MDL Number MFCD00004679
SMILES C(C(CO)(CO)N)O
Synonym TRIS
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3
11

Thermo Scientific™ Tris(hydroxymethyl)aminomethane, Electrophoresis Grade, 99.9%

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 2-Amino-2-hydroxymethyl-1,3-propanediol; TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
MDL Number MFCD00004679
SMILES C(C(CO)(CO)N)O
Synonym 2-Amino-2-hydroxymethyl-1,3-propanediol; TRIS
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3
12

Invitrogen™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats

Promotions are available
EDGE
For Use With (Equipment) Bolt
Concentration 20X
Product Line Bolt
14

Tricine, Electrophoresis Grade, MP Biomedicals

CAS: 5704-04-1 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO

PubChem CID 79784
CAS 5704-04-1
Molecular Weight (g/mol) 179.17
ChEBI CHEBI:39063
SMILES C(C(=O)O)NC(CO)(CO)CO
IUPAC Name 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
InChI Key SEQKRHFRPICQDD-UHFFFAOYSA-N
Molecular Formula C6H13NO5
15

Thermo Scientific Chemicals TRIS-glycine native sample buffer (4X), pH 8.6

For preparation of protein samples for native gel electrophoresis

Color Blue
Physical Form Liquid
pH 8.6
Chemical Name or Material Sample Loading Buffer
Concentration 4X
For Use With (Application) Preparation of protein samples for native gel electrophoresis