
Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Substrate | Horseradish Peroxidase |
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Form | Liquid |
Product Type | Substrate |
Signal Duration | 6 to 8 hr. |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ Pierce™ Streptavidin Poly-HRP
Amplify signal and improve detection of biotinylated antibodies for IHC and other methods using this streptavidin conjugated with HRP polymers.

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate
Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Substrate | Horseradish Peroxidase |
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Sensitivity | Mid-femtogram |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 24 hr. |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate
SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

Shipping Condition | Wet Ice |
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Substrate | Horseradish Peroxidase |
Sensitivity | Low-femtogram to high-attogram |
Form | Liquid |
Product Type | Substrate |
Membrane Compatibility | Nitrocellulose, PVDF |
For Use With (Application) | Western Blot |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

Shipping Condition | Room Temperature |
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Substrate | Horseradish Peroxidase |
Recommended Antibody Concentrations | 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL) |
Conjugate | HRP |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 6 to 24 hr. |
For Use With (Application) | Western Blot |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ Pierce™ 1-Step Transfer Buffer
Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

Product Type | Transfer Buffer |
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Thermo Scientific™ Pierce™ High Sensitivity NeutrAvidin™-HRP
Specially prepared peroxidase-conjugated NeutrAvidin protein for maximum signal amplification with high signal-to-noise ratio in a ready-to-use solution.

Specificity | Biotin |
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Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2°C to 8°C. |
Target Molecule | Biotin |
Form | Liquid |
Enzyme | HRP (Horseradish Peroxidase) |
Label or Dye | HRP (Horseradish Peroxidase) |
For Use With (Application) | Western Blotting, ELISA |
Product Line | Pierce™ |
Detection Method | Colorimetric, Chemiluminescence, Chemifluorescence |
Invitrogen™ NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)
All buffers are made with high-purity reagents and are strictly quality controlled

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate
Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Substrate | Horseradish Peroxidase |
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Content And Storage | Upon receipt store at 2-8°C. Product is shipped at ambient temperature. |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 1 to 2 hr. |
Product Line | Pierce™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Invitrogen™ Bolt™ Antioxidant
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Invitrogen™ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled
No. of Reactions | 10 midi blots or 20 mini blots |
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Product Type | Flex Solution Kit |
For Use With (Equipment) | iBind Flex Western Device |
Membrane Compatibility | PVDF, Nitrocellulose |
Product Line | iBind™ |
Detection Method | Colorimetric, Chromogenic, Chemiluminescence |
Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Sensitivity | Low picogram to mid-femtogram |
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Form | Liquid |
Product Type | Substrate |
Membrane Compatibility | Nitrocellulose, PVDF |
Product Line | Pierce™ |
Detection Method | Chemiluminescence, Fluorescence |
Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Shipping Condition | Dry Ice |
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Description | The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins |
Product Type | Protein Labeling Reagent |
For Use With (Equipment) | Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager |
Membrane Compatibility | Nitrocellulose, PVDF |
Green Features | Less hazardous |
Shelf Life | At least 2 years from receipt |
Run Time | 10 minutes |
For Use With (Application) | Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
Product Line | Invitrogen™ |