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Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Brand: Invitrogen™ A44449
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Description
- Flexible use labeling format—Use with any protein gel of choice to perform total protein labeling of the membrane post-transfer. Or use it as a fast protein stain after electrophoresis of gels you do not intend to transfer.
- Easy to use protocol—Mix and incubate with the transferred membrane. The reaction time is 10 minutes. Gel staining is also a 10 minute reaction.
- Flexible visualization—Use a wide-range of imagers with UV, Green LED or Fluorescence (∼488 mm) light source, including the iBright FL1500 Imaging System, to capture an image.
- Accurate total protein normalization—Designed to provide accurate total protein normalization over a broad linear range for protein detection: 1–80 μg total protein loaded per well.
- Sensitive and stable signal—Protein bands are detected down to 20 ng and the signal is compatible with downstream antibody detection.
- Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions.
Specifications
| Protein Labeling Reagent | |
| The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins | |
| A standard kit will label 40 mini gels or 40 mini gel-sized membranes | |
| Dry Ice | |
| Any Gel Type and Gel Chemistry | |
| Invitrogen™ | |
| Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
| • No-Stain activator, 800 μL, store at -20°C • No-Stain derivatizer, 800 μL, store at -20°C • No-Stain labeling buffer (20X), 2 x 20 mL, store at 15°C to 30°C |
|
| Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager | |
| 10 minutes | |
| Chemiluminescence, Fluorescence | |
| PVDF, Nitrocellulose | |
| 40 reactions |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 40RXN No-Stain Protein Labelling Reagent
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
Hazard Statement
- H319-Causes serious eye irritation.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P370+P378-In case of fire: Use dry sand, dry chemical or alcohol resistant foam to extinguish.
- P403-Store in a well-ventilated place.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Mandelonitrile, DMSO
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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