Protein Purification Reagents and Kits
Protein Purification Reagents and Kits
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Filtered Search Results
Thermo Scientific™ POROS™ 20 OH Hydroxyl Activated Resin
Thermo Scientific™ POROS™ Immobilized Hydroxyl Affinity Bulk Media. POROS OH is based on an hydroxylated functional group designed for the immobilization of proteins through activation with tresyl-chloride, divinyl-sulphone or cyanogen bromide.
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Cytiva PreScission™ Protease
Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.
Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Ligand Type | N-benzyl-n-methyl Ethanolamine |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | HiTrap Capto adhere column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Easy screening and convenient process development |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Stationary Phase | Divalent Nickel, Metal Chelate |
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Type | Resin |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Description | HisPur Ni-NTA Resin |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin |
Product Line | HisPur™ |
Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells
Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.
Thermo Scientific™ Detergent Removal Spin Columns & Plates
Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.
Stationary Phase | Proprietary Ligand |
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Column Type | Proprietary Resin, Affinity |
Purification Target | Detergent Removal |
Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit
Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.
Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.
Buffer | Lysis Buffer |
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Shipping Condition | Ambient |
Form | Liquid |
Product Type | Nuclear and Cytoplasmic Extraction Reagent |
Reagent Type | Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation |
Product Line | NE-PER™ |
Thermo Scientific™ HisPur™ Ni-NTA Spin Plates
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 3 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ HisPur™ Cobalt Resin
Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.
Stationary Phase | Metal Chelate, Divalent Cobalt |
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Type | Resin |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Protein Form | Recombinant |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Product Line | HisPur™ |