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Protein Purification Reagents and Kits

Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1 – 15 of 1,068 results
1

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Promotions are available
EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
2

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

Promotions are available
EDGE
3

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Promotions are available
EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
4

Cytiva PreScission™ Protease

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

Promotions are available
EDGE
5

Cytiva HiTrap™ Capto™ Adhere Columns

Designed to remove contaminants in post-protein A purification

EDGE
Particle Size 75μm
Buffer Aqueous Buffer
Ligand Type N-benzyl-n-methyl Ethanolamine
Height (Metric) 25mm
Content And Storage 4° to 30°C
pH Range 3 to 12
Product Type HiTrap Capto adhere column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Easy screening and convenient process development
Support Type Highly Cross-linked Agarose
Height (English) 0.98 in.
6

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Promotions are available
EDGE
Stationary Phase Divalent Nickel, Metal Chelate
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Ni-NTA Resin
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
Product Line HisPur™
7

Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells

Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.

Promotions are available
EDGE
8

Thermo Scientific™ Detergent Removal Spin Columns & Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.

Promotions are available
EDGE
Stationary Phase Proprietary Ligand
Column Type Proprietary Resin, Affinity
Purification Target Detergent Removal
9

Invitrogen™ Ni-NTA Agarose

Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

Promotions are available
EDGE
Type Agarose
Stationary Phase Ni-NTA
Content And Storage The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.
Form Suspension
Column Type Affinity Column
10

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

Promotions are available
11

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 3 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Promotions are available
EDGE
12

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Promotions are available
Buffer Lysis Buffer
Shipping Condition Ambient
Form Liquid
Product Type Nuclear and Cytoplasmic Extraction Reagent
Reagent Type Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
Product Line NE-PER™
13

Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit

Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.

Promotions are available
14

Thermo Scientific™ HisPur™ Cobalt Resin

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

Promotions are available
Stationary Phase Metal Chelate, Divalent Cobalt
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Cobalt Resin
Protein Form Recombinant
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Product Line HisPur™
15

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Promotions are available
Stationary Phase Divalent Nickel, Metal Chelate
Type Agarose
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) 20 mg His-Tagged GFP Binding per mL Resin
Product Line HisPur™