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Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

€ 197.00 - € 2343.00

Specifications

Diameter (English) Inner 0.6 in.
Diameter (Metric) Inner 16 mm
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate <20 mL/min.
Material Polypropylene
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10126414
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Cytiva
17-5319-01
5 x 1 mL
€ 197.00
Pack of 5
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11595674
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Cytiva
17-5319-02
100 x 1 mL This item is currently unavailable or has been discontinued.
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Description

Description

  • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
  • Column material: polypropylene
  • Bed dimensions: 16 × 25mm
  • Flow velocity: <20mL/min.
  • Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
  • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
  • Simple operation with syringe, perstaltic pump, or chromatographic system
  • Columns may be connected in series to increase capacity

Medium
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

Specifications

Specifications

0.6 in.
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Polypropylene
6% Cross-linked Agarose
5 bar (0.5 MPa, 70 psi)
16 mm
<20 mL/min.
3 to 12
4°C to 30°C, 20% Ethanol
Pre-packed Column
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Cytiva HisTrap™ FF Columns: 16 x 25mm

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