A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.
Brand: Thermo Scientific™ PV4529
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a synthetic peptide substrate for NDR1.
| Z'-LYTE™ Ser/Thr 19 Peptide Kinase Assay Kit, 800 x 20μL, 800rxns, Liquid, Serine/Threonine Kinase Assay Type, Peptide-Based Substrate, 535nm Emission, 405/465nm Excitation, Methylcoumarin Label, End Point Readout, Serine/Threonine Kinases Target Enzyme, FRET Technique, Used for Pharma and Biopharma, Protein Assays and Analysis | |
| Liquid | |
| Z'-LYTE™ Ser/Thr 11 peptide, Z'-LYTE™ Ser/Thr 11 phospho-peptide, 5X kinase buffer A, ATP, development reagent B, development buffer | |
| Serine/Threonine Kinase Assay | |
| –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. | |
| 800 x 20μL, 800 x 20 μL Assays | |
| 405/465, 535 nm | |
| 800 Reactions | |
| EMBO J. vol. 17, pp. 5913-22 (1998) | |
| Based on Synthetic Peptide Substrate for NDR1 (KKRNRRLSVA) | |
| AURKA, CHEK1 | |
| AURKA, CHEK1 |
| Z ́-LYTE™ | |
| Pharma and biopharma, protein biology, drug discovery and development, target and lead identification and validation, kinase biology, biochemical kinase assays, z and acute-lyte™ kinase assays, enzyme and protein activity assays, protein assays and, FRET | |
| 800 x 20μL | |
| Biochemical phospho-specific activity (no antibody) | |
| Z'-LYTE™ Ser/Thr 19 Peptide Kinase Assay Kit | |
| 384-well plate | |
| Methylcoumarin | |
| End Point | |
| Dry Ice | |
| Peptide-Based Substrate | |
| Serine/Threonine Kinases |
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For Research Use Only. Not for use in diagnostic procedures.
