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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 15 Peptide
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.
Brand: Thermo Scientific™ PV3799
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Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon EGFR, containing Thr-693.
Specifications
| Z'-LYTE™ Kinase Assay Kit - Ser/Thr 15 Peptide, 800 x 20μL Assays, 800 rxns, 405/465nm Excitation, 535nm Emission, Methylcoumarin Label, FRET Technique, Serine/Threonine Kinases Target Enzyme, Peptide-Based Substrate Properties, End Point Readout, | |
| Cell viability, proliferation and function, pharma and biopharma, protein assays and analysis, target and lead identification and validation, FRET | |
| 800 x 20μL | |
| Biochemical phospho-specific activity (no antibody) | |
| Z'-LYTE™ Kinase Assay Kit - Ser/Thr 15 Peptide | |
| 384-well plate | |
| Methylcoumarin | |
| End Point | |
| Signal Transduction | |
| EGFR residues 688-702, centered on Thr-693 (LVEPLTPSGEAPNQK) | |
| MAPK11 | |
| MAPK11 |
| Z ́-LYTE™ | |
| All necessary reagents for performing primary or secondary screens | |
| Serine/Threonine Kinase Assay | |
| –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. | |
| 800 x 20μL, 800 x 20 μL | |
| 405/465, 535 nm | |
| 800 Reactions | |
| J. Cell. Phys.(1995), v. 162, p434 | |
| Dry Ice | |
| Peptide-Based Substrate | |
| Serine/Threonine Kinases |
For Research Use Only. Not for use in diagnostic procedures.