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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells

€ 333.00 - € 445.00

Specifications

Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Cloning Method TOPO™-TA
Content And Storage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Format Kit
Product Line TOPO™
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15751927
View Documents
Invitrogen™
452640
10 Reactions
€ 333.00
Each
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11503837
View Documents
Invitrogen™
450640
25 Reactions
€ 445.00
25 reactions
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Description

Description

Includes the pCR™II-TOPO TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable cloning method, featuring a 5-minute cloning reaction and 3-step protocol, and yielding up to 95% recombinants. Resistant to Kanamycin (KanR) andZeocin™ (ZeoR)
Kit Options: TOPO TA Cloning Dual Promoter Kits
TOPO TA Cloning Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:

  • General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40)
  • High-efficiency cloning: TOP10 Electrocomp™ cells (Cat. Nos. K4660-01, K4660-40)
  • General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. Nos. K4620-01, K4620-40)
  • Fast growth: Mach1™ -T1R chemically competent E. coli (Cat. No. K4610-20)
  • Repressor/induction needs: TOP10F' cells (Cat. Nos. K4650-01, K4650-40)
  • Provide your own cells (Cat. Nos. 450640 and 452640)

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and SP6 promoters for efficient in vitro transcription
  • M13 forward and reverse primer sites for sequencing
  • 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
  • Kanamycin and ampicillin resistance for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants

Cloning, PCR Cloning

Specifications

Specifications

Ampicillin (AmpR), Kanamycin (KanR)
Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
TOPO™
TOPO-TA Vectors
TOPO™-TA
Kit
T7, SP6
Videos
Safety and Handling

Safety and Handling

missing translation for 'shelfLife' : All reagents are stable for 6 months when properly stored.

SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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