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Applied Biosystems™ TaqMan™ Copy Number Reference Assay, human, RNase P
Human TaqMan™ Copy Number Reference Assays are run with human TaqMan™ Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome.
€ 185.00 - € 528.00
Specifications
| 3'Primer Modification | None |
|---|---|
| 5'Primer Modification | None |
| Content And Storage | Control Primer-Probe Set(s) |
| Detection Method | Primer-probe |
| Form | Frozen |
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|
10535585
|
Applied Biosystems™
4403326 |
750 Reactions |
€ 185.00
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
|
10514055
|
Applied Biosystems™
4403328 |
3000 Reactions |
€ 528.00
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
- Assays are run with human TaqMan™ Copy Number Assays in a duplex real-time PCR reaction
- Used to detect and measure copy number variations (CNVs) and smaller regions in the human genome
- Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT
- Designed to unique genomic sequences in the reference genome assembly (build GRCh38) and are required for relative quantitation of copy number targets
- TaqMan™ Copy Number Assays quantitate the gene of interest and normalize to an endogenous reference gene known to be present in two copies in a diploid genome
- Please note that TaqMan™ Copy Number Reference Assays are species specific.
- Assays should be stored at -15°C to -25°C in the dark
- Minimize freeze-thaw cycles
TaqMan™ Copy Number Reference Assay RNase P
- Recommended as the standard reference assay for copy number analysis
- This assay detects the Ribonuclease P RNA component H1 (H1RNA) gene (RPPH1) on chromosome 14, cytoband 14q11.2
- The assay location is chr.14:20343370 on build GRCh38
- It has an 87 bp amplicon that maps within the single exon RPPH1 gene
TaqMan™ Copy Number Reference Assay TERT
- An alternative reference assay recommended in the event that the RNase P assay functions poorly with a sample
- This could be due to chromosomal aberrations or other issues
- This assay targets the telomerase reverse transcriptase (TERT) gene located on chromosome 5, cytoband 5p15.33
- The assay location is chr5:1253257 on build GRCh38
- It has an 88 bp amplicon that maps within exon 16 of the TERT gene
Specifications
| None | |
| Control Primer-Probe Set(s) | |
| Frozen | |
| Low | |
| Standard | |
| Sustainable packaging | |
| VIC | |
| Configurable Assay | |
| RNase-Free, DNase-Free | |
| Human | |
| 20X |
| None | |
| Primer-probe | |
| Tube | |
| qPCR | |
| RPPH1 | |
| TAMRA™ Quencher (3'), VIC™ (5') | |
| TaqMan™ | |
| HPLC | |
| Ambient Temperature | |
| RNase P |
For Research Use Only. Not for use in diagnostic procedures.
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