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Thermo Scientific™ Tango™ PTAFR-bla U2OS Cells
The Tango™ PTAFR-bla U2OS cells contain the human Platelet Activating Factor Receptor (PTAFR) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Brand: Thermo Scientific™ K1789
Description
The Tango™ PTAFR-bla U2OS cells contain the human Platelet Activating Factor Receptor (PTAFR) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ PTAFR-bla U2OS cells are functionally validated for Z' and EC50 concentrations of β-Acetyl-γ-O-hexadecyl-L-α-phosphatidylcholine. In addition, Tango™ PTAFR-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
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Specifications
NM_000952 | |
Dividing Cells | |
Native Tail | |
FRET | |
PTAFR-bla U2OS cells, PTAFR-bla U2OS Assay Protocol, Certificate of Analysis | |
GeneBLAzer™ | |
End Point | |
PTAFR | |
U2OS | |
GPCR |
Cell-based beta-arrestin recruitment | |
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ PTAFR-bla U2OS cells • Tango™ PTAFR-bla U2OS Assay Protocol • Certificate of Analysis The Tango™ PTAFR-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
Fluorescence | |
PTAFR | |
Pool of Stable Cells | |
8 x 106 Cells | |
BLA (Beta-Lactamase) | |
Dividing Cells | |
Tango™ |
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For Research Use Only. Not for use in diagnostic procedures.