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Thermo Scientific™ Tango™ HTR1D-bla U2OS Cells
The Tango™ HTR1D-bla U2OS cells contain the human 5-Hydroxytryptamine (Serotonin) Receptor 1D (HTR1D) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Brand: Thermo Scientific™ K1524
Description
The Tango™ HTR1D-bla U2OS cells contain the human 5-Hydroxytryptamine (Serotonin) Receptor 1D (HTR1D) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ HTR1D-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ HTR1D-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.
Specifications
NM_000864 | |
Dividing Cells | |
Tail from AVPR2 GPCR | |
Fluorescence | |
HTR1D | |
Single Cell Clone Origin | |
2 x 106 Cells | |
U2OS | |
HTR1D | |
U2OS | |
GPCR |
Cell-based beta-arrestin recruitment | |
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ HTR1D-bla U2OS cells • Tango™ HTR1D-bla U2OS Assay Protocol • Certificate of Analysis The Tango™ HTR1D-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
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Tango™ HTR1D-bla U2OS Cells, 2 x 106 Cells, U2OS Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage | |
Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays | |
Tango™ HTR1D-bla U2OS cells (LiveBLAzer™-FRET B/G loading kit, solution D and other materials are required separately; please refer to the protocol). Includes: Tango™ HTR1D-bla U2OS cells, Tango™ HTR1D-bla U2OS assay protocol, certificate of analysis | |
Tango™ | |
BLA (Beta-Lactamase) | |
Room Temperature | |
U2OS Cell | |
Tango™ |
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For Research Use Only. Not for use in diagnostic procedures.