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Thermo Scientific™ Tango™ HTR1A-bla U2OS Cells
Description
Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. Tango™ HTR1A-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of 5-CT (Figure 1). In addition, Tango™ HTR1A-bla U2OS cells have been tested for assay performance under variable conditions.
Specifications
Specifications
| Accession Number | NM_000524 |
| Assay Entry | Cell-based beta-arrestin recruitment |
| Cell State | Dividing Cells |
| Content And Storage | LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ HTR1A-UAS-bla CHO-K1 cells • Tango™ HTR1A-UAS-bla CHO-K1 Assay Protocol • Certificate of Analysis The Tango™ HTR1A-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
| Cytoplasmic Tail | Tail from AVPR2 GPCR |
| Detection Method | Fluorescence |
| For Use With (Application) | FRET |
| Gene Symbol | HTR1A |
| Human Protein Class | G-Protein Coupled Receptor (GPCR) |
| Includes | HTR1A-UAS-bla CHO-K1 cells, HTR1A-UAS-bla CHO-K1 Assay Protocol, Certificate of Analysis |
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For Research Use Only. Not for use in diagnostic procedures.
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