Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent

Description

Perform mild extraction of total protein from tissue samples with Thermo Scientific™ T-PER Tissue Protein Extraction Reagent.

This tissue cell lysis reagent utilizes a proprietary detergent in 25mM bicine and 150mM sodium chloride (pH 7.6) to maximize the efficiency of protein solubilization from mammalian tissue samples by homogenization. The simple composition is compatible with additives such as protease inhibitors, salts, reducing agents and chelating agents, providing versatility for many different sample types and lysis applications. Cell lysates prepared with T-PER Reagent are directly compatible with reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures.

Highlights:

• Simple procedure – just homogenize tissue sample in 1:20 (w/v) of tissue to T-PER Reagent, then centrifuge to pellet cell/tissue debris

• Mild and convenient – detergent is dialyzable for quick and easy removal; can be used with additional components (e.g., protease inhibitors, salts, reducing agents, chelating agents, etc.)

• Flexible – lysate may be used for reporter assays, protein kinase assays, immunoassays and/or protein purifications

• Compatible – the lysate is compatible with standard protein assays such as Thermo Scientific Pierce™ Coomassie Plus™ (Bradford) Protein Assay