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Molecular Probes™ Single-Channel Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 and SYTOX™ Green Dyes, for flow cytometry
Single Channel Dual 384-Well Temperature Verification Kit for PCR 9700 System, 0.02mL
Brand: Molecular Probes™ V13240
This item is not returnable.
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Description
This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent Alexa Fluor™ 488 dye and dead cells using SYTOX™ Green nucleic acid stain. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show brighter green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished in the FL1 channel of a flow cytometer, freeing the other channels for detection of other fluorescent colors.View a selection guide for all apoptosis assays for flow cytometry.
Specifications
| Tube | |
| Contains 1 vial of annexin V, Alexa Fluor™ 488 conjugate (250 μL), 1 vial of SYTOX™ green (100 μL), and 1 bottle of annexin binding buffer (5X solution, 50 mL). Store in refrigerator (2–8°C) and protect from light. |
|
| SYTOX™ green: 503⁄524, Alexa Fluor™ 488: 499⁄521 | |
| Wet Ice | |
| Flow Cytometry | |
| Flow Cytometer |
| Dead Cell Apoptosis Kit | |
| 488 | |
| 50 | |
| Alexa Fluor™, SYTOX™ | |
| Alexa Fluor 488, SYTOX Green |
For Research Use Only. Not for use in diagnostic procedures.