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Thermo Scientific Chemicals Safranine O

Product Code. 11349729
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Quantity:
10 g
50 g
This item is not returnable. View return policy
This item is not returnable. View return policy

It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.

Solubility
Soluble in water at 20°C, 50g/L. Soluble in alcohol.

Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 477-73-6
Molecular Formula C20H19ClN4
Molecular Weight (g/mol) 350.85
MDL Number MFCD00011759
InChI Key QRYAEWIQIBAZOJ-UHFFFAOYSA-N
Synonym basic red 2, safranine o, gossypimine, safranin, safranine t, safranin o, safranin t, safranine, tolusafranine, hidaco safranine
PubChem CID 2723800
IUPAC Name 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine;chloride
SMILES [Cl-].CC1=C(N)C=C2C(=C1)N=C1C(C)=C(N)C=CC1=[N+]2C1=CC=CC=C1

Specifications

Odor Odorless
Quantity 10 g
Beilstein 3924099
Solubility Information Soluble in water at 20°C,50g/L. Soluble in alcohol.
Formula Weight 350.85
Chemical Name or Material Safranine O
compliance-icons
Product Identifier
  • Safranine O
Signal Word
  • Warning
Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Supplemental information
  • MIXTURE LIST-Contains : Safranine O

RUO – Research Use Only

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