Invitrogen™ Retinol Binding Protein ELISA Kit

Description

The Retinol Binding Protein Competitive ELISA quantitates retinol binding protein (RBP) in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.∣Principle of the method: The Retinol Binding Protein Competitive ELISA research-use-only kit is designed to quantitatively measure RBP independent of species. An RBP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-sheep IgG antibodies. Anti-sheep RBP antibodies and RBP-peroxidase conjugate is added to the wells. The RBP-peroxidase conjugate and any RBP in the sample will compete to bind to the anti-sheep RBP antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound RBP-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of RBP present in the sample.∣Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene.