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R&D Systems™ Recombinant Human Flt-3 Ligand/FLT3L Fc (HEK293) Protein
Brand: R&D Systems™ 11309-FKH-250
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Description
Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3. The ED50 for this effect is 0.300-4.80 ng/mL.Specifications
AAA17999.1 | |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. | |
M.W. Observed: 57-64 kDa, under reducing conditions., M.W. Theoretical: 45 kDa | |
Human embryonic kidney cell, HEK293-derived human Flt-3 Ligand/FLT3L protein Human Flt-3 Ligand/FLT3L (Thr27-Pro185) (N-terminus) Accession # AAA17999.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus) | |
<0.10 EU per 1 μg of the protein by the LAL method. | |
Unconjugated | |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Bioactivity | |
2323 | |
250 μg | |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. | |
FL, FLG3L, Flt3 ligand, Flt3L, FLT3LG, fms-related tyrosine kinase 3 ligand, SL cytokine | |
Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS. | |
Flt-3 Ligand/FLT3L |
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