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R&D Systems™ Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Brand: R&D Systems™ 4114-TC-01M
896.74 EUR valid until 2024-12-16
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This item is not returnable.
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Description
This protein was used at 20-50 ng/mL to support a 100-fold expansion of rat cortical neural stem cells or mouse cortical stem cells. Cells were cultured in N2 plus medium and supplemented with fresh FGF basic/FGF2/bFGF every day. Medium was changed every other day and cells were split every 2-4 days upon becoming 70% confluent. Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.3-3 ng/mL.Specifications
| Bioactivity | |
| 2247 | |
| FGF basic 145 aa, Tissue Culture Grade | |
| E. coli-derived human FGF basic/FGF2/bFGF protein Ala144-Ser288 | |
| Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin | |
| Recombinant |
| Lyophilized from a 0.2μm filtered solution in Tris-HCl and NaCl. | |
| 16 kDa | |
| 1 mg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution. | |
| Unconjugated | |
| Reconstitute at 0.5-1.0 mg/mL in sterile PBS. |