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Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit
Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit is two-component, high-precision, detergent-compatible assay optimized to measure (at 480 nm) total protein concentration compared to protein standard curve of known concentrations.
Brand: Thermo Scientific™ A53225
Alert:
Store at 4°C
Description
- A fast, two-component, high-precision, detergent-compatible assay optimized to determine total protein concentration
- Can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications
- Assay maintains the key characteristics of the traditional BCA assay but allows a fast time and room temperature incubation equal to dye-binding methods
- Provides comparable accuracy but with a 5 min., room temperature incubation and measured at 480 nm
- This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results
- Exhibits similar protein-to-protein variation as traditional BCA Protein Assay and less protein-to-protein variation than dye-binding protein assay methods (Bradford)
- Unaffected by typical concentrations of most ionic and non-ionic detergents
- Linear working range for BSA of 20 to 2000 μg/mL
- Store in refrigerator at 2°C to 8°C
Specifications
| 20 to 2000 μg/mL | |
| Rapid Gold BCA Assay | |
| Pierce™ | |
| Not Target-Specific | |
| Spectrophotometer, Microplate Reader | |
| Wet Ice |
| Store in refrigerator (2–8°C). | |
| Colorimetric | |
| Protein Quantitation Assay | |
| Solution-based Detection, Absorbance | |
| 500 mL |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Pierce Rapid Gold BCA Protein Assay Kit
Signal Word
- Warning
Hazard Category
- LONG-TERM AQUATIC HAZARD Chronic 2
- ACUTE AQUATIC Acute 1
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H319-Causes serious eye irritation.
- H400-Very toxic to aquatic life.
- H411-Toxic to aquatic life with long lasting effects.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P233-Keep container tightly closed.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P242-Use only non-sparking tools.
- P243-Take precautionary measures against static discharge.
- P264-Wash hands thoroughly after handling.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P391-Collect spillage.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : acetonitrile, copper(II) sulphate pentahydrate
For Research Use Only. Not for use in diagnostic procedures.