Rabbit anti-Sheep IgG (H+L) Secondary Antibody, Biotin, Invitrogen™

Description

Product # 31840 has been successfully used in Western blot, IF, ICC, IHC and ISH applications. Product # 31840 reacts with the heavy chains of sheep IgG and with the light chains common to most sheep immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody cross-reacts with bovine IgG, goat IgG (50%) sheep IgM (6%) bovine IgM, goat IgM (3%) guinea pig IgG, horse IgG (1%) and it has minimum cross-reactivity with immunoglobulins from other species.

Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.