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Invitrogen™ PUMA alpha Polyclonal Antibody
Rabbit Polyclonal Antibody
Brand: Invitrogen™ PA534755
This item is not returnable.
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Description
Recommended positive controls: K562, THP-1, HCT116 control 0hr, HCT116 treated 30uM cisplatin 24hr. IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
Specifications
| PUMA alpha | |
| Polyclonal | |
| Unconjugated | |
| BBC3 | |
| BBC3; BCL2 binding component 3; Bcl-2 binding component 3; bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; BH3-only; FLJ42994; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; PUMA; PUMA/JFY1 | |
| Rabbit | |
| Antigen affinity chromatography | |
| RUO | |
| 27113 | |
| Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
| Liquid |
| Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot | |
| 1 mg/mL | |
| 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7 | |
| Q9BXH1 | |
| BBC3 | |
| Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PUMA. The exact sequence is proprietary. | |
| 100 μL | |
| Primary | |
| Human | |
| Antibody | |
| IgG |
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