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  5. Invitrogen™ Mouse Progranulin (GRN) ELISA Kit

Invitrogen™ Mouse Progranulin (GRN) ELISA Kit

Product Code. p-200020554
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Quantity:
10 x 96 Tests
96 Tests
Unit Size:
1 set
Pack of 10
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Product Code. 18507824

Brand: Invitrogen™ EMGRNX10

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Description

ELISA

The Mouse Progranulin (GRN) ELISA quantitates Ms Progranulin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Progranulin. Principle of the method The Mouse Progranulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Progranulin is the precursor protein of Granulin also called as Proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature Granulin. Both Granulin and smaller cleavage product of Granulin regulates cell growth. Progranulin stimulates proliferation of many epithelial cells, promotes their anchorage-independent growth and, when overexpressed, confers epithelial tumorigenicity. Progranulin/epithelin gene is multifunctional, with important constitutive roles in epithelial homeostasis, reproductive, immunological, and neuronal function. Progranulin constitutively expressed in a number of epithelia, particularly in the skin, GI tract, and reproductive system. Progranulin is expressed in immune cells in vivo and in specific neurons in the brain.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P28798
Assay Range 20.58 to 15,000 pg/mL
Assay Sensitivity 20 pg/mL
Calibration Range 20 pg/mL to 15000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Transcription Factors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, O x 40, Ligand, P-Cadherin, Periostin, PlGF-2, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon.
Sufficient For 96 Assays
Target Species Mouse
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 14824
Gene Symbol Grn
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Research Discipline Development
Sample Volume Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym RP23-226P4.4, Pgrn, epithelin
Target Progranulin
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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