Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Brand: Thermo Scientific™ 34035
Thermo Scientific™ Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).Highlights:
AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph
Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor
1-Step™ NBT/BCIP
1-Step NBT/BCIP Plus Suppressor
| Pierce NBT Substrate | |
| Colorimetric | |
| Nitrocellulose, PVDF | |
| Alkaline Phosphatase | |
| Upon receipt store product at 4°C protected from moisture. Product is shipped at ambient temperature. |
| Substrate | |
| Powder | |
| 1 g | |
| Alkaline Phosphatase |
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| ZA4098221 | Certificate of Analysis | 02/02/2024 | 34035 |
| YC35103670 | Certificate of Analysis | 09/03/2023 | 34035 |
| SPECS | Certificate of Analysis | 10/08/2022 | 34035 |
| WA3084853 | Certificate of Analysis | 06/01/2021 | 34035 |
| VF29167513 | Certificate of Analysis | 10/06/2020 | 34035 |
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
For Research Use Only. Not for use in diagnostic procedures.
