Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay.
Brand: Thermo Scientific™ 88282
The Thermo Scientific™ Pierce LAL Chromogenic Endotoxin Quantitation Kit measures the amount of endotoxin in a protein, peptide or antibody sample using the Limulus Amebocyte Lysate (LAL) assay.
The endotoxin concentration in a sample is measured using the Pierce LAL Chromogenic Endotoxin Quantitation Kit via a chromogenic signal generated in the presence of endotoxins. Samples can be measured on a microplate absorbance reader at 405nm. A standard curve is created using the E. coli endotoxin standard included with each kit to calculate endotoxin levels as low as 0.1 EU/mL. Protein and antibody samples can be assayed in as little as 30 minutes. Determining endotoxin levels is important to assess the efficiency of endotoxin removal methods and prevent endotoxic shock, inflammation and/or sepsis in tissue culture cells and animals injected with endotoxin-contaminated proteins.
Highlights:
Sensitive—detect as little as 0.1 EU/mL
Fast—perform this assay in 30 minutes
Economical—assay requires only 10μL of a protein sample
Accurate—E. coli O111:B4 standard in each kit enables accurate endotoxin quantitation
Versatile—405nm absorbance reading is compatible with common ELISA plate readers
Recommended for:
Quantitation of endotoxin levels in a protein, peptide or antibody solution
| Upon receipt store at 4°C. Product shipped with an ice pack. | |
| Colorimetric | |
| Pierce™ | |
| Solution-Based Detection, Absorbance | |
| Endotoxin Quantitation Kit |
| [other] | |
| Spectrophotometer, Microplate Reader | |
| 50 Tests | |
| Not Target-Specific | |
| Kit |
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