Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters
Brand: Thermo Scientific™ 16176
95.33 EUR valid until 2025-03-21
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The Thermo Scientific™ Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than two hours) in the presence of firefly luciferase reporters for protein expression assays.
This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of firefly luciferase in cell lysates. In the presence of firefly luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Firefly Luc Plasmids; however, this assay kit may be used to detect the activity of other firefly or ATP-dependent luciferases that use D-luciferin as a substrate.Highlights:
Sensitive – highly sensitive detection of firefly luciferase activity; for best results (maximum signal) use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer
Stable – increased signal stability compared to flash assays
Simple – does not require a luminometer with injectors
Compatible – assay reagents compatible with other firefly beetle luciferases
Automation-friendly – amenable to high throughput assays
Convenient – contains a universal cell lysis buffer and optimized glow assay reagent
Safe – allows one to perform non-radioactive assays
Includes:
Cell lysis buffer, reaction buffer and substrate
Requires:
Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; For best results (maximum signal), use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer
Recommended for:
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
| Assay Kit | |
| 100 Reaction Kit | |
| Reporter Enzyme, Luciferase Reporter Assay | |
| Bioluminescence | |
| 384-well plate, 96-well plate | |
| Chemical Substrate | |
| Luciferase Substrate |
| Luciferase, Firefly Luciferase | |
| • Firefly Glow Assay Buffer, 5 mL, store at -20°C • D-Luciferin, Lyophilized, 3 mg, store at 4°C • 2X Cell Lysis Buffer, 6 mL, store at room temperature Upon receipt store kit at -20°C or store individual components as indicated above. |
|
| Mammalian Cells | |
| Luminometer (Microplate) | |
| Enzyme Labeled | |
| Enhanced Chemiluminescence | |
| D-Luciferin |
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For Research Use Only. Not for use in diagnostic procedures.
