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Thermo Scientific™ Pierce™ Bradford Protein Assay Kit

Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Brand: Thermo Scientific™ 23200

142.67 EUR valid until 2025-03-21
Use promo code "24111" to get your promotional price.

Product Code. 10270014

€ 214.00 / 1 set

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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price

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Description

Measure protein concentration using a stable formulation of the original Bradford reagent described in the literature with Thermo Scientific™ Pierce Coomassie Protein Assay Kit.

The Pierce Coomassie Protein Assay Kit is a ready-to-use formulation of the popular assay reagent originally described by Bradford in 1976. When mixed with a protein solution, the acidic Coomassie-dye reagent changes color from brown to blue in proportion to the amount of protein present in the sample. Protein determinations are made by comparison to the color response of protein assay standards, usually prepared as a series of known dilutions of bovine serum albumin (BSA) or bovine gamma globulin (BGG). The kit includes Coomassie Protein Assay Reagent and a package of Albumin Standard Ampules. The simple procedure is adaptable to nearly any volume scale, including test tubes, cuvets and microplates.

Highlights:

Bradford reagent – stable, ready-to-use kit of the classical Bradford assay reagent
Colorimetric – measure with a standard spectrophotometer or plate reader (595nm)
Easy to use – single reagent; no working reagent preparation required
Fast – almost immediate color development; add, mix and read results
Broad range – detects protein concentration in the range 1 to 1500μg/mL
Flexible – microplate and cuvet protocols provided with the instructions and adaptable to several target working ranges

Includes:

Coomassie Protein Assay Reagent and 10 ampules of Albumin Standard

Specifications

Content And Storage	Sufficient For: 630 tube assays or 3800 microplate assays Pierce Bradford Assay Reagent, 950 mL Albumin Standard ampules, 2 mg/mL, 10 x 1 mL Store Bradford Protein Assay Reagent at 4°C. Store unopened Albumin Standard ampules at room temperature.
Detection Method	Colorimetric
Product Type	Protein Quantitation Assay
Sufficient For	630 Tube Assays or 3800 Microplate Assays
For Use With (Equipment)	Spectrophotometer, Microplate Reader

Assay	Bradford Assay
Product Line	Pierce™
Specificity	Not Target-Specific
For Use With (Application)	Solution-based Detection, Absorbance
Quantity	950 mL

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

Pierce Coomassie (Bradford) Protein Assay Kit

Signal Word

Danger

Hazard Category

Acute toxicity Category 4
Skin corrosion/irritation Category 1 B
Serious eye damage/eye irritation Category 1
Specific target organ toxicity Category 2

Hazard Statement

H302-Harmful if swallowed.
H312-Harmful in contact with skin.
H332-Harmful if inhaled.
H314-Causes severe skin burns and eye damage.
H318-Causes serious eye damage.
H371-May cause damage to organs.

Precautionary Statement

P280-Wear protective gloves/protective clothing/eye protection/face protection.
P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
P331-Do NOT induce vomiting.
P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
P305-IF IN EYES:.
P405-Store locked up.
P501-Dispose of contents/container to .

Supplemental information

MIXTURE LIST-Contains : phosphoric acid, methanol

Documents

Scientific Resources

Application Notes

Tech Tip: Protein quantitation assay compatibility table File size: 104 kB

Tech Tip: How to use a protein assay standard curve File size: 65.6 kB

Tech Tip: Quantitate immobilized protein File size: 39.4 kB

Product Information

Manuals

User Guide: Pierce Bradford Protein Assay Kit Version: C.0 File size: 353.9 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot NumberZL402129	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog Number23200
Lot NumberZJ400242	Certificate TypeCertificate of Analysis	Date16/12/2024	Catalog Number23200
Lot NumberZI399159	Certificate TypeCertificate of Analysis	Date15/10/2024	Catalog Number23200
Lot NumberZI394927	Certificate TypeCertificate of Analysis	Date15/10/2024	Catalog Number23200
Lot NumberZG394928	Certificate TypeCertificate of Analysis	Date16/08/2024	Catalog Number23200

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Thermo Scientific™ Pierce™ Bradford Protein Assay Kit > Kit; 950mL kit

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