Thermo Scientific™ Pierce™ CDI-activated Agarose Resin

Description

Thermo Scientific™ Pierce CDI-Activated Agarose is a porous, beaded chromatography support that is activated with carbonyldiimidazole groups for covalent immobilization of amine-containing affinity ligands.

Pierce CDI-activated Agarose is a porous, beaded chromatography support that is activated with carbonyl diimidazole groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles. Coupling can be performed in aqueous or organic solvent conditions, making possible the immobilization of many kinds of ligands. Once a molecule is immobilized, the prepared affinity resin can be used multiple times in typical affinity purification procedures.

Highlights:

• Reliable coupling chemistry – immobilization occurs through the reaction of N-nucleophiles with 1,1'-carbonyldiimidazole groups of the resin to form stable, uncharged N-alkylcarbamate linkages

• Easy to use – no secondary reagents needed; just wash equilibrate resin in alkaline coupling buffer and add ligand; reaction proceeds spontaneously

• Stable activation – half-life of hydrolysis is much longer than hydroxysuccinimide ester activations, making immobilization reactions easier to prepare and control; simplifies filtration and washing before adding a ligand or protein

• Well-defined coupling condition – reaction is most efficient with primary amines at pH 9 to 11

Properties of CDI-Activated Agarose:

• Resin: crosslinked 6% beaded agarose
• Activation: at least 50μmol 1,1'-carbonyldiimidazole (CDI) groups per milliliter of resin
• Format: supplied as stabilized, 50% slurry in acetone