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Macherey-Nagel™ NucleoSpin™ miRNA, Mini kit for miRNA and RNA purification
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No. of Reactions:
10 Reactions
250 Reactions
50 Reactions
Unit Size:
10 reactions
250 reactions
50 reactions
Description
Features
- Parallel isolation of small and large RNA
- RNA purification fractionated by size:
- Isolation of small RNA only (< 200 b),
- Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
- Isolation of total RNA (small and large RNA in one fraction)
- Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
- Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform (patent pending)
- NucleoSpin™ Filters for efficient sample homogenization
- rDNase for efficient on-column removal of genomic DNA
Applications
- Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with phenol / chloroform (e.g., TRIzol™) lysis
- Purification of siRNA and large dsRNA from DICER reactions
- Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization
Specifications
Specifications
| Format | Mini Spin Column |
| For Use With (Application) | Purification of siRNA and large dsRNA from DICER reactions |
| Includes | NucleoSpin™ RNA Columns, NucleoSpin™ miRNA Columns, NucleoSpin™ Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin™ Filters, buffers, RNase-free rDNase |
| No. of Reactions | 50 Reactions |
| Product Type | NucleoSpin™ miRNA Kit |
| RNase | Yes |
| Sample Size | Small RNA: < 200 nt, large RNA: > 200 nt |
| Test Time | <45 min./6 Reaction and <35 min./6 Reaction |
| Yield | 10μg small RNA, 95μg large RNA |
Safety and Handling
Signal Word
- Danger
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 2
- LONG-TERM AQUATIC HAZARD Chronic 3
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 2
- Flammable liquid Category 3
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H225-Highly flammable liquid and vapour.
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H335-May cause respiratory irritation.
- H351-Suspected of causing cancer.
- H411-Toxic to aquatic life with long lasting effects.
- H412-Harmful to aquatic life with long lasting effects.
- P201-Obtain special instructions before use.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
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