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Invitrogen™ TNF alpha Human ELISA Kit, Ultrasensitive
Quantifies natural and recombinant human Tumor Necrosis Factor Alpha (TNF-α) in serum, plasma, buffered solution, or cell culture medium
€ 712.00 - € 2145.00
Specifications
| Accession Number | P01375 |
|---|---|
| Assay Range | 0.5-32 pg/mL |
| Assay Sensitivity | <0.09 pg/mL |
| Conjugate | Biotin |
| Product Type | ELISA Kit |
Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
10465133
|
Invitrogen™
KHC3014 |
96 Tests |
€ 712.00
96 tests |
Estimated Shipment: 05-12-2024
Log in to see stock. |
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|
10160573
|
Invitrogen™
KHC3013 |
2 x 96 Tests |
€ 1150.00
192 tests |
Estimated Shipment: 02-12-2024
Log in to see stock. |
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|
10475133
|
Invitrogen™
KHC3014C |
5 x 96 Tests |
€ 2145.00
Pack of 5 |
Estimated Shipment: 02-12-2024
Log in to see stock. |
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Description
The Human Tumor Necrosis Factor Alpha (Hu TNFα) ELISA quantitates Hu TNFα in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFα. Principle of the method The Human TNFα ultrasensitive solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
TNF-α is produced by macrophages and has many immune effects. Ultrasensitive ELISA Kits are approximately 10X more sensitive than the conventional ELISA Kit.
Apoptosis, Apoptosis Related Factors, Cell Analysis, Cell Signaling, Cell Viability, Proliferation and Function, Immunoassays, Ready-To-Use Immunoassay
Order Info
Shipping Condition: Wet ice
Specifications
| P01375 | |
| <0.09 pg/mL | |
| ELISA Kit | |
| Human | |
| Colorimetric Microplate Reader | |
| DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF | |
| 5.9% | |
| HRP | |
| RUO | |
| 2°C to 8°C | |
| 1 hr. 20 min. |
| 0.5-32 pg/mL | |
| Biotin | |
| Plasma, Serum, Supernatant | |
| Colorimetric | |
| 7124 | |
| 8.5% | |
| Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| <0.09pg/mL | |
| Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
| Human | |
| 3 hr. |
For Research Use Only. Not for use in diagnostic procedures.