The Dynabeads™ Co-Immunoprecipitation Kit is intended for the coupling of an antibody to the surface of Dynabeads™ M-270 Epoxy beads. The coupled beads and supplied buffers can then be used for co-immunoprecipitation (co-IP) of proteins, intact protein complexes, or intact protein-nucleic acid complexes. In addition to epoxy-activated Dynabeads magnetic beads, the kit includes the buffers needed for efficient antibody coupling, gentle washing, and elution of the co-isolated protein complexes.
Benefits of Dynabeads Co-Immunoprecipitation Kit include: • Convenience—contains optimized buffers and epoxy-activated Dynabeads magnetic beads for efficient co-IP • Stabile—gentle separation technology preserves native protein conformations and large protein complexes • Flexibility—allows coupling of any antibody of your choice • Robustness—covalent antibody coupling to the Dynabeads magnetic beads avoids co-elution of the antibody with the target proteins
Efficient and easy coupling With this kit, antibodies are covalently coupled to Dynabeads M-270 Epoxy beads, minimizing the risk of bound antibodies contaminating the final eluate. Dynabeads M-270 Epoxy beads exhibit ultra-low background binding and do not require blocking before use. The supplied buffers are optimized to give consistent and reliable results. The kit includes eight different binding, washing, and elution buffers, in addition to Dynabeads M-270 Epoxy beads, to enable purification of complex protein structures of any size. All kit components are analytical grade and compatible with protease and phosphatase inhibitors. Target specific antibody is required for coupling, but not included. The protocol includes coupling, sample preparation (detergent and cryolysis methods), co-IP, and elution and is compatible with western blot/silver stain, Coomassie stain, mass spectrometry, and extraction of nucleic acids from isolated DNA/RNA binding protein complexes.
Magnetic bead–based separation offers easy handling Captured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag magnet due to the magnetic properties of the Dynabeads M-270 Epoxy beads. The kit is designed for and tested with cultured yeast and mammalian cells and is compatible with tissue, insect, bacterial, and other lysates.
Automation-ready for use with KingFisher purification systems The 2.8-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher sample purification systems
Commercial supply Our manufacturing sites are ISO 13485–certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility, making them an ideal choice for commercial supply. If you are in the process of customizing the Dynabeads Co-Immunoprecipitation Kit in a commercial product/service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or read more on our Dynabeads OEM page
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