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Invitrogen™ Neon™ Transfection System 100 μL Kit
Neon™ Transfection System 100 μL Kit
Brand: Invitrogen™ MPK10096
Includes:
30mL Resuspension Buffer R, 30mL Resuspension Buffer T, 2 x 150mL Electrolytic Buffer E2, 96 x 100μL Neon™ Tips, 20 Neon Electroporation Tubes
Description
- For use with the Neon Transfection System
- Use this kit for transfection volumes of 100μL, containing 5 x 105-2 x 106 adherent cells or 1 x 106-5 x 106 suspension cells
- Cells that have been transfected using the included 100μL Neon Tips are then ready to be washed and plated into a 6-well, 60mm, or 10cm culture dish, depending on protocol
Adenoviral shRNA, Cell Culture, Lentiviral shRNA & miRNA, Plasmid Transfection, RNAi, RNAi Transfection, RNAi, Epigenetics & Noncoding RNA Research, Retroviral RNAi, Stem Cell & Primary Cell Transfections, Synthetic siRNA Transfection, Transfection, Vector-Based RNAi, siRNA
Order Info
Shipping Condition: Room Temperature
Specifications
| Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum | |
| Stem Cells, Primary Cells, Hard-to-Transfect Cells | |
| Plasmid DNA, Synthetic siRNA | |
| 5 x 10^5 - 2 x 10^6 cells/mL (Adherent Cells), 1 x 10^6 - 5 x 10^6 cells/mL | |
| Transfection | |
| Neon™ | |
| 96 x 2 Reactions | |
| Room Temperature | |
| Neon™ Transfection System |
| • 30 mL Resuspension Buffer R • 30 mL Resuspension Buffer T • 2 x 150 mL Electrolytic Buffer E2 • 96 x 100 μL Neon™ Tips • 20 Neon™ Electroporation Tubes Store the buffer box 4°C and the tips & tubes box at room temperature. |
|
| 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks | |
| Electroporation | |
| Neon™ Transfection System | |
| 96 x 2 | |
| Transfection System Kit | |
| Yes | |
| 2x10^4 - 6 x10^8 cells; Varies by cell type | |
| 100μl |
For Research Use Only. Not for use in diagnostic procedures.