Gibco™ Mouse EGF Native Protein

Description

Reconstitution: Reconstituted EGF should be aliquoted and stored in polypropylene vials and is stable at -5°C to -20°C for up to two months. Avoid repeated freezing and thawing. Stability of dilute solutions of EGF may be prolonged by addition of bovine serum albumin to a final concentration of 1.0%. This natural preparation of murine epidermal growth factor (EGF) was isolated from male mouse submaxillary glands. It is not sterile as provided.

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.