Invitrogen™ Staphylococcus aureus (Wood strain without protein A) BioParticles™, fluorescein conjugate

• Fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities
• Fluorescent BioParticles products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry
• Fluorescein-labeled BioParticles conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor™, BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformLy intense over the pH range from 3 to 10
• Label (Ex/Em): Fluorescein (approx. 494/518nm)
• Particle: S. aureus (Wood strain, without protein A)
• Provided as lyophilized powders
• Approximately 3 x 10⁸ S. aureus particles per mg solid and approximately 2 x 10⁷ zymosan particles per mg solid
• Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
• Fluorescence of BioParticles conjugates that are bound to surface of cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
• Addition to cellular applications, fluorescent BioParticles conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutant s
• Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Cell Analysis, Cell Structure, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Receptors