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Invitrogen™ DetectaGene™ Green CMFDG lacZ Gene Expression Kit
For a membrane-impermeant, peptide-fluorescent dye adduct
Brand: Invitrogen™ D2920
This item is not returnable.
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Description
The DetectaGene™ Green lacZ Gene Expression Kit includes a fluorescein-based β-galactose derivative that has been chemically modified to include a mildly thiol-reactive chloromethyl group. Once inside a cell, the DetectaGene substrate undergoes two reactions: 1) its two galactose moieties are cleaved by intracellular β-galactosidase and 2) either simultaneously or sequentially, its chloromethyl moiety reacts with glutathione and possibly other intracellular thiols to form a membrane-impermeant, peptide-fluorescent dye adduct.
Beta-Galactosidase Assays, Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Flow Cytometry, Flow Cytometry Antibodies and Secondary Detection, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Proteins, Expression, Isolation and Analysis, Reporter Assays
Order Info
Shipping Condition: Room Temperature
Specifications
| β-Galactosidase Reporter Gene Assay System | |
| β-Gal (lacZ) Assay | |
| Room Temperature | |
| Beta-Gal Substrate | |
| Beta-Galactosidase | |
| DetectaGene |
| Fluorescence | |
| Store in freezer (-5 to -30°C) and protect from light. | |
| CMFDG | |
| Chemical Substrate | |
| Kit | |
| 1 kit |
For Research Use Only. Not for use in diagnostic procedures.