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Molecular Probes™ Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry
Vybrant™ Apoptosis Assay Kit #13 *PO-PRO™-1/7-aminoactinomycin D* *for flow cytometry* *200 assays*
Brand: Molecular Probes™ V35123
This item is not returnable.
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Description
This flow cytometry product detects apoptosis on the basis of changes that occur in the permeability of cell membranes using PO-PRO™-1 and 7-aminoactinomycin D (7-AAD) nucleic acid stains. After staining a cell population with PO-PRO™-1 dye and 7-AAD, apoptotic cells show violet fluorescence, dead cells show violet and red fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished by a flow cytometer that uses both a violet laser and the 488 nm line of an argon-ion laser for excitation. Each kit contains sufficient reagents for approximately 200 flow cytometry tests.View a selection guide for all apoptosis assays for flow cytometry.
Specifications
| Tube, Slide | |
| Contains 1 vial of PO-PRO™-1 dye (500 μL), and 1 vial of 7-AAD (7-aminoactinomycin D, 200 μL). Store in refrigerator (2–8°C) and protect from light. |
|
| PO-PRO™-1: 434⁄456, 7-AAD: 546⁄647 | |
| Room Temperature | |
| Flow Cytometry | |
| Fluorescence Microscope, Flow Cytometer |
| Dead Cell Apoptosis Kit | |
| 405, 488 | |
| 200 | |
| Vybrant™ | |
| 7-AAD, PO-PRO-1 |
For Research Use Only. Not for use in diagnostic procedures.