Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR

Description

The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. It provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. These cDNA Synthesis kits use Maxima Reverse Transcriptase (RT), an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features high thermostability, robustness and increased cDNA synthesis rate compared to wild type M-MuLV RT.

The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is capable of reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 μg) at elevated temperatures (42–65°C). The synthesis reaction can be completed in 15–30 minutes. Components of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR are pre-mixed to save time and to reduce the possibility of pipetting errors.

The Maxima First Strand cDNA Synthesis Kit for RT-qPCR with dsDNase also contains a novel double-strand specific DNase engineered to remove contaminating genomic DNA from RNA preps in two minutes without damage to quality or quantity of RNA. Highly specific activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.

Features of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR include:

• High yields of full length cDNA up to 20 kb
• Efficient cDNA synthesis at wide temperature range (42–65°C)
• Increased synthesis rate—complete cDNA synthesis in 15–30 minutes
• High sensitivity and specificity
• Integrated gDNA removal step with dsDNase kit

Maxima First Strand cDNA Synthesis Kit with dsDNase contains:

• Maxima Enzyme Mix
• 5X Reaction Mix
• nuclease-free water

Kit with dsDNase also contains

• dsDNase
• 10X dsDNase Buffer

Applications

• 2-step RT-PCR
• 2-step RT-qPCR

Additional information about reaction components

The Maxima Enzyme Mix contains Maxima Reverse Transcriptase and RiboLock RNase Inhibitor. The recombinant RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B, and C at temperatures up to 55°C.

5X Reaction Mix contains the remaining reaction components: reaction buffer, dNTPs, oligo(dT)18 and random hexamer primers. Nuclease-free water is provided for reaction set-up and dilution of sample RNA.

The absence of endo- and exo-deoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.