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Invitrogen™ Lyo-ready Bst DNA Polymerase

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.

€ 66.50 - € 274.00

Specifications

Product Type Stand-alone enzyme
For Use With (Application) LAMP, RT-LAMP
Polymerase Lyo-ready Bst DNA Polymerase
Optimal Reaction Temperature 65°C
Shipping Condition Dry Ice
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Products 3
Product Code Brand Concentration Quantity Price Quantity & Availability  
Product Code Brand Concentration Quantity Price Quantity & Availability  
17815017
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Invitrogen™
A56655
6 U/μL 1200 U
€ 66.50
1200 units
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17805217
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Invitrogen™
A56656
6 U/μL 6000 U
€ 274.00
6000 units
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17885207
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Invitrogen™
A56657
40 U/μL 1200 U
€ 66.50
1200 units
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).

Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.

Lyo-ready Bst DNA Polymerase features
• Fast target detection in as little as 10 minutes
• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction
• Robust amplification, even from inhibitor-containing RNA or DNA samples
• Ability to optimize LAMP or RT-LAMP reactions to fit your needs

Applications
Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Recombinase polymerase amplification (RPA)
• Helicase-dependent amplification (HDA)
• Exponential amplification reaction (EXPAR)

Notes
1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.
2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.

Specifications

Specifications

Stand-alone enzyme
Lyo-ready Bst DNA Polymerase
Dry Ice
LAMP, RT-LAMP
65°C
≤10 minutes
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