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Invitrogen™ Lyo-ready Bst DNA Polymerase
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.
€ 66.50 - € 274.00
Specifications
Product Type | Stand-alone enzyme |
---|---|
For Use With (Application) | LAMP, RT-LAMP |
Polymerase | Lyo-ready Bst DNA Polymerase |
Optimal Reaction Temperature | 65°C |
Shipping Condition | Dry Ice |
Product Code | Brand | Concentration | Quantity | Price | Quantity & Availability | ||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Concentration | Quantity | Price | Quantity & Availability | ||||
17815017
|
Invitrogen™
A56655 |
6 U/μL | 1200 U |
€ 66.50
1200 units |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
17805217
|
Invitrogen™
A56656 |
6 U/μL | 6000 U |
€ 274.00
6000 units |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
17885207
|
Invitrogen™
A56657 |
40 U/μL | 1200 U |
€ 66.50
1200 units |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Description
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.
Lyo-ready Bst DNA Polymerase features
• Fast target detection in as little as 10 minutes
• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction
• Robust amplification, even from inhibitor-containing RNA or DNA samples
• Ability to optimize LAMP or RT-LAMP reactions to fit your needs
Applications
Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Recombinase polymerase amplification (RPA)
• Helicase-dependent amplification (HDA)
• Exponential amplification reaction (EXPAR)
Notes
1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.
2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.
Specifications
Stand-alone enzyme | |
Lyo-ready Bst DNA Polymerase | |
Dry Ice |
LAMP, RT-LAMP | |
65°C | |
≤10 minutes |