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Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector, without competent cells
Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector
€ 352.00 - € 626.00
Specifications
| Bacterial or Yeast Strain | Not Included |
|---|---|
| Cloning Method | TA Cloning |
| Content And Storage | This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Format | Kit |
| For Use With (Application) | PCR Cloning |
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|
10266302
|
Invitrogen™
K207040 |
40 Reactions |
€ 626.00
Each |
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|
10442832
|
Invitrogen™
K207020 |
20 Reactions |
€ 352.00
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
The T7 and Sp6 promoters of the pCRII vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCRII cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.
- Fast and convenient: 15-minute, room-temperature ligation
- Efficient: blue/white screening and >80% clones with correct insert
- Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
- Hassle-free: eliminates any enzymatic modifications of PCR product
- Streamlined: does not require use of PCR primers that contain restriction sites
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- T7 and Sp6 promoters for in vitro RNA transcription and sequencing
- Versatile polylinker with flanking EcoR I sites for easy excision of inserts
- M13 forward and reverse primer sites for sequencing
- Cloning Method: Restriction Enzyme/MCS
- How TA Cloning Works
- Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
- Linearized vector supplied in this kit has single 3' deoxythymidine (T) residues
- Allows PCR inserts to ligate efficiently with vector
- Kit Configurations
- TA Cloning Kit is offered in a variety of configurations: with One Shot INVF' Chemically Competent E. coli (K2050-01 and K2050-40), One Shot TOP10F' Chemically Competent E. coli (K2060-01 and K2060-40), and without competent cells (K2750-20 and K2750-40) in 20- and 40-reaction kit sizes
Cloning, PCR Cloning
Specifications
| Not Included | |
| This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
|
| PCR Cloning | |
| Cloning Kit | |
| pCRII |
| TA Cloning | |
| Kit | |
| TA Cloning™ | |
| T7, SP6 |
For Research Use Only. Not for use in diagnostic procedures.
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