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Invitrogen™ PureLink™ HiPure Plasmid Miniprep Kit
The PureLink HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without use of any organic solvents or cesium chloride, and contains Low endotoxin levels.
Brand: Invitrogen™ K210002
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Description
The PureLink HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without use of any organic solvents or cesium chloride, and contains Low endotoxin levels.
Plasmid DNA of High Purity
- PureLink HiPure Plasmid Miniprep Kit is designed to efficiently isolate plasmid DNA from E.coli in 1.5–2 hours
- Kit employs anion-exchange resin composed of small particles with a uniform pore size to purify plasmid DNA to a level equivalent to two passes through CsCl gradients
- Need for additional steps to remove contaminants such as phenol, chloroform, and CsCl are eliminated, minimizing exposure to and disposal of hazardous materials
- Efficient—Increased yields with low endotoxin Levels
High Yield and Efficient Endotoxin Removal
- PureLink HiPure Plasmid Miniprep Kit allows purification of up to 30 μg of high quality plasmid DNA from 1–3 mL overnight E.coli cultures in approximately 1 hour, with an endotoxin level of 0.1–1 EU/μg, when cloning high copy number plasmids
- Typically, DNA isolated using the PureLink HiPure Plasmid Miniprep Kit has an A260/A280 >1.80 when samples are diluted in Tris-HCl (pH 7.5) indicating that the DNA is reasonably free of proteins that could interfere with downstream applications
Isolated DNA can be used for a Number of Downstream Applications:
- Purified DNA is suitable for downstream applications, including those requiring the highest purity DNA, such as mammalian transfections, automated and manual DNA sequencing, PCR amplification, in vitro transcription, bacterial cell transformation, cloning, and labeling
Order Info
Shipping Condition: Room Temperature
Specifications
| • 10 mL Resuspension Buffer (R3) • 100 μL RNase A • 10 mL Lysis Buffer (L7) • 10 mL Precipitation Buffer (N3) • 50 mL Equilibration Buffer (EQ1) • 125 mL Wash Buffer (W8) • 25 mL Elution Buffer (E4) • 15 mL TE Buffer • 25 HiPure Columns Store all components at room temperature. |
|
| Anion Exchange Resin | |
| Bacterial Culture | |
| Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription | |
| 25 Preps | |
| PureLink™ | |
| 25 Preps | |
| BAC DNA, Plasmid DNA |
| Kit | |
| Mini | |
| BAC DNA, Plasmid DNA | |
| Not High-throughput Compatible (Manual) | |
| < 100 μg (Small-Scale) Plasmid DNA | |
| Plasmid MiniPrep Kit | |
| Room Temperature | |
| 2 hr. |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- PureLink HiPure Plasmid Miniprep Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
Supplemental information
- MIXTURE LIST-Contains : Sodium lauryl sulfate, sodium hydroxide
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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