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Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit

The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli.

€ 235.00 - € 408.00

Specifications

Format Column
Isolation Technology Anion Exchange Resin
Sample Type Bacterial Culture
Elution Volume 5 mL
Final Product Type BAC DNA, Plasmid DNA
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Products 2
Product Code Brand Includes Quantity Price Quantity & Availability  
Product Code Brand Includes Quantity Price Quantity & Availability  
10083762
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Invitrogen™
K210004
25 Preps
€ 235.00
1191mL
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10388702
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Invitrogen™
K210005
50 Preps
€ 408.00
50 reactions
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Description

Description

The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350 μg plasmid DNA from 15–25 mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

  • Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
  • High-purity, low-endotoxin product- pure enough for mammalian cell transfection
  • Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification

  • PureLink HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to level equivalent to two passes through CsCl gradients
  • The resin combines excellent capacity with fast flow rate, high resolution, high yield, and efficient endotoxin removal
  • Plasmid preparations can typically be completed in less than 2 hours

Contaminant-free DNA

  • Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
  • Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of greater than 1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
  • Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
  • Purification time is two hours

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Column
Bacterial Culture
BAC DNA, Plasmid DNA
Not High-throughput Compatible (Manual)
100-200 μg (Medium-Scale) Plasmid DNA
Plasmid MidiPrep Kit
Room Temperature
PureLink™
350 μg
Anion Exchange Resin
5 mL
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
PureLink™
Transfection Grade
BAC DNA, Plasmid DNA
2 hr.
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Safety and Handling

Safety and Handling

Product Identifier
  • PureLink HiPure Plasmid Midiprep Kit

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.

Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.

Supplemental information
  • MIXTURE LIST-Contains : acetic acid, Sodiumlauryl sulfate, Sodium hydroxide

SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.