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Invitrogen™ pIZT/V5-His Vector Kit

Product Code. 10236542
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Quantity:
1 kit
Unit Size:
1 set
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Product Code. 10236542

Brand: Invitrogen™ V801001

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Description

Includes

The InsectSelect Glow Kit includes 20μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2μg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1g Zeocin, Cellfectin Reagent, Sf9 cells, and 1 L of GIBCO Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above.

For high-level expression of the gene of interest

  • The OpIE2 promoter for constitutive expression
  • The Zeocin resistance gene for rapid selection of stably transfected cell lines
  • C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Anti-V5 Antibody and rapid purification with nickel-chelating resin
  • In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin resistance protein (Zeo-GFP)
  • The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin and confers a fluorescent phenotype that simplifies identification of transfected cells

Constitutive Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Promoter OplE2
Product Type Insect Cell Expression Vector
Content And Storage The InsectSelect™ Glow Kit includes 20 μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 μg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin™ Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Protein Tag Position (to your gene) C-terminal
Protein Tag His Tag (6x), V5 Epitope Tag
Cloning Method Restriction Enzyme/MCS
Quantity 1 kit
Vector pIZ
Product Line InsectSelect

For Research Use Only. Not for use in diagnostic procedures.

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