missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ pBAD/gIII Kit
Express recombinant proteins under the tight regulation of the araBAD promoter
Brand: Invitrogen™ V45001
1301.04 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Includes: T20μg each of pBAD/gIII A, B and C, 20μg of pBAD/gIII/calmodulin, 1mL 20% L-arabinose, LMG194 and TOP10 E. coli stabs.
Description
- To simplify purification, vector pBAD/gIII encodes a leader peptide which directs recombinant protein into the periplasmic space
- Features araBAD promoter for tightly regulated expression
- Translation initiation signals for optimized E. coliexpression
- Leader peptide from bacteriophage fd gene III protein (gIII) for secreted expression
- C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
- C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
- A set of three vectors is provided (A, B, and C): Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of the gene
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| araBAD | |
| None | |
| Ampicillin (AmpR) | |
| Restriction Enzyme/MCS | |
| pBAD |
| Arabinose | |
| Bacterial Expression Vector | |
| The pBAD/gIII Kit includes 20 μg each of pBAD/gIII A, B, & C, 20 μg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored. | |
| c-Myc Epitope Tag | |
| 1 kit |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction