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Invitrogen™ InsectSelect™ Glow Kit with Sf9 Cells
Features the pIZT/V5-His vector for high-level expression of the gene of interest
Brand: Invitrogen™ K81001
3166.80 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Includes: The InsectSelect Glow Kit includes 20μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2μg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1g Zeocin, Cellfectin Reagent, Sf9 cells, and 1L of GIBCO Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described.
Description
- The pIZT/V5-His vector features the OpIE2 promoter for constitutive expression
- The Zeocin resistance gene for rapid selection of stably transfected cell lines
- C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Anti-V5 Antibody and rapid purification with nickel-chelating resin
- pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin resistanc protein (Zeo-GFP): The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin and confers a fluorescent phenotype that simplifies identification of transfected cells
Constitutive Protein Expression in Insect Cells, High-level Protein Production in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| OplE2 | |
| The InsectSelect™ Glow Kit includes 20 μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 μg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin™ Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored. | |
| His Tag (6x), V5 Epitope Tag | |
| 1 kit | |
| InsectSelect™ |
| Sf9 Cells Glow Kit | |
| C-terminal | |
| Restriction Enzyme/MCS | |
| pIZ |
For Research Use Only. Not for use in diagnostic procedures.